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map 1b  (Proteintech)


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    Proteintech map 1b
    Map 1b, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 19 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/map 1b/product/Proteintech
    Average 93 stars, based on 19 article reviews
    map 1b - by Bioz Stars, 2026-05
    93/100 stars

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    Netrin-1 stimulation changes MAP localization. (A) Representative images of <t>MAP1B</t> in neurons at DIV1 after fixation, Scale Bar 10 μm. (B) MAP1B in the soma changes with Netrin-1 after 10 min ( N = 9 or more neurons from 2 animals). (C) MAP1B changes in the axon after 10 min of Netrin-1 stimulation ( N = 9 or more neurons from 2 animals). (D) MAP1B increases in the growth cone following Netrin-1 stimulation. (E) Representative images of DCX in DIV1 neurons after fixation, Scale Bar 10 μm. (F) Doublecortin does not change in the soma following Netrin-1 stimulation ( N = 9 or more neurons from 2 animals). (G) Doublecortin does increase in the axon following Netrin-1 stimulation for 10 min ( N = 9 or more neurons from 2 animals). (H) Doublecortin trends towards an increase in the growth cone following 10 min of Netrin-1 stimulation ( N = 9 or more neurons from 2 animals).
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    Netrin-1 stimulation changes MAP localization. (A) Representative images of <t>MAP1B</t> in neurons at DIV1 after fixation, Scale Bar 10 μm. (B) MAP1B in the soma changes with Netrin-1 after 10 min ( N = 9 or more neurons from 2 animals). (C) MAP1B changes in the axon after 10 min of Netrin-1 stimulation ( N = 9 or more neurons from 2 animals). (D) MAP1B increases in the growth cone following Netrin-1 stimulation. (E) Representative images of DCX in DIV1 neurons after fixation, Scale Bar 10 μm. (F) Doublecortin does not change in the soma following Netrin-1 stimulation ( N = 9 or more neurons from 2 animals). (G) Doublecortin does increase in the axon following Netrin-1 stimulation for 10 min ( N = 9 or more neurons from 2 animals). (H) Doublecortin trends towards an increase in the growth cone following 10 min of Netrin-1 stimulation ( N = 9 or more neurons from 2 animals).
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    Figure 4. Microtubule-associated protein 1B <t>(MAP1B)</t> expression in the rostral migratory stream (RMS). (A) Immunohistochemistry of the RMS showing MAP1B expression (magenta) along the stream. The RMS is delineated with dotted lines. Scale bar: 50 μm. (B) Immunostaining for MAP1B of a neuroblast migrating away from a ventricular/subventricular zone (V/SVZ) explant in Matrigel. The labeling is located around the nucleus and in the leading process with occasional bundles of potential microtubules (arrow). Scale bar: 10 μm.
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    Image Search Results


    Journal: Cell Death & Disease

    Article Title: Opposing roles for GSK3β and ERK1-dependent phosphorylation of huntingtin during neuronal dysfunction and cell death in Huntington’s disease

    doi: 10.1038/s41419-025-07524-0

    Figure Lengend Snippet:

    Article Snippet: Mouse anti-MAP1B , Santa Cruz Biotechnology , Cat# sc-365668 RRID: AB_10847224.

    Techniques: Transduction, Recombinant, Protease Inhibitor, Magnetic Beads, Plasmid Preparation, In Situ, Software, Imaging

    Netrin-1 stimulation changes MAP localization. (A) Representative images of MAP1B in neurons at DIV1 after fixation, Scale Bar 10 μm. (B) MAP1B in the soma changes with Netrin-1 after 10 min ( N = 9 or more neurons from 2 animals). (C) MAP1B changes in the axon after 10 min of Netrin-1 stimulation ( N = 9 or more neurons from 2 animals). (D) MAP1B increases in the growth cone following Netrin-1 stimulation. (E) Representative images of DCX in DIV1 neurons after fixation, Scale Bar 10 μm. (F) Doublecortin does not change in the soma following Netrin-1 stimulation ( N = 9 or more neurons from 2 animals). (G) Doublecortin does increase in the axon following Netrin-1 stimulation for 10 min ( N = 9 or more neurons from 2 animals). (H) Doublecortin trends towards an increase in the growth cone following 10 min of Netrin-1 stimulation ( N = 9 or more neurons from 2 animals).

    Journal: Frontiers in Neuroscience

    Article Title: Netrin-1 stimulated axon growth requires the polyglutamylase TTLL1

    doi: 10.3389/fnins.2024.1436312

    Figure Lengend Snippet: Netrin-1 stimulation changes MAP localization. (A) Representative images of MAP1B in neurons at DIV1 after fixation, Scale Bar 10 μm. (B) MAP1B in the soma changes with Netrin-1 after 10 min ( N = 9 or more neurons from 2 animals). (C) MAP1B changes in the axon after 10 min of Netrin-1 stimulation ( N = 9 or more neurons from 2 animals). (D) MAP1B increases in the growth cone following Netrin-1 stimulation. (E) Representative images of DCX in DIV1 neurons after fixation, Scale Bar 10 μm. (F) Doublecortin does not change in the soma following Netrin-1 stimulation ( N = 9 or more neurons from 2 animals). (G) Doublecortin does increase in the axon following Netrin-1 stimulation for 10 min ( N = 9 or more neurons from 2 animals). (H) Doublecortin trends towards an increase in the growth cone following 10 min of Netrin-1 stimulation ( N = 9 or more neurons from 2 animals).

    Article Snippet: Mouse MAP1B Santa Cruz Biotech Cat#: sc-135978.

    Techniques:

    TTLL1 OE alters Netrin-1 induced changes in MAP localization. (A) Representative images of MAP1B in TTLL1 OE neurons at DIV1 after fixation, Scale Bar 10 μm. (B) MAP1B in the soma does not change with Netrin-1 after 10 min ( N = Minimum of 5 neurons from 2 animals). (C) MAP1B does not change in the axon after 10 min of Netrin-1 stimulation ( N = Minimum of 5 neurons from 2 animals). (D) MAP1B continues to increase in the growth cone following Netrin-1 stimulation in TTLL1 OE neurons ( N = Minimum of 5 neurons from 2 animals). (E) Representative images of DCX in DIV1 TTLL1 OE neurons after fixation, Scale Bar 10 μm. (F) Doublecortin does not change in the soma following Netrin-1 stimulation ( N = Minimum of 5 neurons from 2 animals). (G) Doublecortin does not increase in the axon following Netrin-1 stimulation for 10 min ( N = Minimum of 5 neurons from 2 animals). (H) Doublecortin does not increase in the growth cone following 10 min of Netrin-1 stimulation ( N = Minimum of 5 neurons from 2 animals).

    Journal: Frontiers in Neuroscience

    Article Title: Netrin-1 stimulated axon growth requires the polyglutamylase TTLL1

    doi: 10.3389/fnins.2024.1436312

    Figure Lengend Snippet: TTLL1 OE alters Netrin-1 induced changes in MAP localization. (A) Representative images of MAP1B in TTLL1 OE neurons at DIV1 after fixation, Scale Bar 10 μm. (B) MAP1B in the soma does not change with Netrin-1 after 10 min ( N = Minimum of 5 neurons from 2 animals). (C) MAP1B does not change in the axon after 10 min of Netrin-1 stimulation ( N = Minimum of 5 neurons from 2 animals). (D) MAP1B continues to increase in the growth cone following Netrin-1 stimulation in TTLL1 OE neurons ( N = Minimum of 5 neurons from 2 animals). (E) Representative images of DCX in DIV1 TTLL1 OE neurons after fixation, Scale Bar 10 μm. (F) Doublecortin does not change in the soma following Netrin-1 stimulation ( N = Minimum of 5 neurons from 2 animals). (G) Doublecortin does not increase in the axon following Netrin-1 stimulation for 10 min ( N = Minimum of 5 neurons from 2 animals). (H) Doublecortin does not increase in the growth cone following 10 min of Netrin-1 stimulation ( N = Minimum of 5 neurons from 2 animals).

    Article Snippet: Mouse MAP1B Santa Cruz Biotech Cat#: sc-135978.

    Techniques:

    Model mechanism showing Netrin-1 stimulation increases polyglutamylation of microtubules over time. This increased polyglutamylation leads to increases in MAP1B and DCX in the axon, which stabilizes the microtubule cytoskeleton. This increased stability allows for improved axon growth following Netrin-1 stimulation.

    Journal: Frontiers in Neuroscience

    Article Title: Netrin-1 stimulated axon growth requires the polyglutamylase TTLL1

    doi: 10.3389/fnins.2024.1436312

    Figure Lengend Snippet: Model mechanism showing Netrin-1 stimulation increases polyglutamylation of microtubules over time. This increased polyglutamylation leads to increases in MAP1B and DCX in the axon, which stabilizes the microtubule cytoskeleton. This increased stability allows for improved axon growth following Netrin-1 stimulation.

    Article Snippet: Mouse MAP1B Santa Cruz Biotech Cat#: sc-135978.

    Techniques:

    Journal: Frontiers in Neuroscience

    Article Title: Netrin-1 stimulated axon growth requires the polyglutamylase TTLL1

    doi: 10.3389/fnins.2024.1436312

    Figure Lengend Snippet:

    Article Snippet: Mouse MAP1B Santa Cruz Biotech Cat#: sc-135978.

    Techniques: Dissection, shRNA, Control, Plasmid Preparation, Modification

    Figure 4. Microtubule-associated protein 1B (MAP1B) expression in the rostral migratory stream (RMS). (A) Immunohistochemistry of the RMS showing MAP1B expression (magenta) along the stream. The RMS is delineated with dotted lines. Scale bar: 50 μm. (B) Immunostaining for MAP1B of a neuroblast migrating away from a ventricular/subventricular zone (V/SVZ) explant in Matrigel. The labeling is located around the nucleus and in the leading process with occasional bundles of potential microtubules (arrow). Scale bar: 10 μm.

    Journal: eLife

    Article Title: FMRP regulates postnatal neuronal migration via MAP1B

    doi: 10.7554/elife.88782

    Figure Lengend Snippet: Figure 4. Microtubule-associated protein 1B (MAP1B) expression in the rostral migratory stream (RMS). (A) Immunohistochemistry of the RMS showing MAP1B expression (magenta) along the stream. The RMS is delineated with dotted lines. Scale bar: 50 μm. (B) Immunostaining for MAP1B of a neuroblast migrating away from a ventricular/subventricular zone (V/SVZ) explant in Matrigel. The labeling is located around the nucleus and in the leading process with occasional bundles of potential microtubules (arrow). Scale bar: 10 μm.

    Article Snippet: Primary antibodies used are: MAP1B (Santa Cruz Biotechnology, sc- 365668, 1/100), Vinculin (Cell Signaling Technology, 13901 S, 1/1000).

    Techniques: Expressing, Immunohistochemistry, Immunostaining, Labeling

    Figure 5. Map1b knockdown (KD) rescues Fmr1-null neurons migration defects. (A) Migration speed of Fmr1-null neurons expressing miRNEG and miRMap1b and control neurons expressing miRNEG. Fmr1-null neurons + miRNEG: 45.23 (23.25) μm/hr; Fmr1-null neurons + miRMap1b: 65.72 (24.31) μm/hr; control neurons + miRNEG: 64.54 (21.99) μm/hr (Kruskall-Wallis test: Chi2=61.168, p-value <0.001, df = 2; followed by Dunn’s post hoc test). (B) Percentage of pausing time of Fmr1-null neurons expressing miRNEG and miRMap1b and control neurons expressing miRNEG. Fmr1-null neurons

    Journal: eLife

    Article Title: FMRP regulates postnatal neuronal migration via MAP1B

    doi: 10.7554/elife.88782

    Figure Lengend Snippet: Figure 5. Map1b knockdown (KD) rescues Fmr1-null neurons migration defects. (A) Migration speed of Fmr1-null neurons expressing miRNEG and miRMap1b and control neurons expressing miRNEG. Fmr1-null neurons + miRNEG: 45.23 (23.25) μm/hr; Fmr1-null neurons + miRMap1b: 65.72 (24.31) μm/hr; control neurons + miRNEG: 64.54 (21.99) μm/hr (Kruskall-Wallis test: Chi2=61.168, p-value <0.001, df = 2; followed by Dunn’s post hoc test). (B) Percentage of pausing time of Fmr1-null neurons expressing miRNEG and miRMap1b and control neurons expressing miRNEG. Fmr1-null neurons

    Article Snippet: Primary antibodies used are: MAP1B (Santa Cruz Biotechnology, sc- 365668, 1/100), Vinculin (Cell Signaling Technology, 13901 S, 1/1000).

    Techniques: Knockdown, Migration, Expressing, Control

    Figure 6. The fragile X messenger ribonucleoprotein (FMRP)/microtubule-associated protein 1B (MAP1B) duo acts on the microtubular nuclear cage of rostral migratory stream (RMS) neurons. (A) Representative control neurons from mirNEG-GFP (magenta) plus DCX-RFP (cyan) co-electroporated ventricular/subventricular zone (V/SVZ) explants cultured in Matrigel displaying microtubular bundles enveloping the nucleus. (B) Representative Fmr1 knockdown (KD) neurons from mirFmr1-GFP (magenta) plus DCX-RFP (cyan) co-electroporated V/SVZ explants cultured in Matrigel. The majority of them display an abnormal cage with disruption (arrows) (46%), sinuous cages (33%), or even detached bundle (arrow) (28%). (C) Representative rescued neurons from mirNEG-GFP (magenta) plus DCX-RFP (cyan) plus mirMAP1B co-electroporated V/SVZ explants cultured in Matrigel displaying microtubular bundles enveloping the nucleus. Scale bars: 5 μm. (D) Quantification of the percentage of disorganized cages in the different conditions: mirNEG 19%, miRFMR1 60%, mirFMR1 + mirMAP1B 28% (Pearson’s χ2 test [2, N=107])=14.16, p-value <0.001 with the Benjamini-Hochberg method for

    Journal: eLife

    Article Title: FMRP regulates postnatal neuronal migration via MAP1B

    doi: 10.7554/elife.88782

    Figure Lengend Snippet: Figure 6. The fragile X messenger ribonucleoprotein (FMRP)/microtubule-associated protein 1B (MAP1B) duo acts on the microtubular nuclear cage of rostral migratory stream (RMS) neurons. (A) Representative control neurons from mirNEG-GFP (magenta) plus DCX-RFP (cyan) co-electroporated ventricular/subventricular zone (V/SVZ) explants cultured in Matrigel displaying microtubular bundles enveloping the nucleus. (B) Representative Fmr1 knockdown (KD) neurons from mirFmr1-GFP (magenta) plus DCX-RFP (cyan) co-electroporated V/SVZ explants cultured in Matrigel. The majority of them display an abnormal cage with disruption (arrows) (46%), sinuous cages (33%), or even detached bundle (arrow) (28%). (C) Representative rescued neurons from mirNEG-GFP (magenta) plus DCX-RFP (cyan) plus mirMAP1B co-electroporated V/SVZ explants cultured in Matrigel displaying microtubular bundles enveloping the nucleus. Scale bars: 5 μm. (D) Quantification of the percentage of disorganized cages in the different conditions: mirNEG 19%, miRFMR1 60%, mirFMR1 + mirMAP1B 28% (Pearson’s χ2 test [2, N=107])=14.16, p-value <0.001 with the Benjamini-Hochberg method for

    Article Snippet: Primary antibodies used are: MAP1B (Santa Cruz Biotechnology, sc- 365668, 1/100), Vinculin (Cell Signaling Technology, 13901 S, 1/1000).

    Techniques: Control, Cell Culture, Knockdown, Disruption